Transfection Reagents
Oz Biosciences POLYMAG NEO STARTING KIT
Starting Kit with Super Magnetic plate + 100 µL of PolyMag Neo, PolyMag + CombiMag
Lonza Walkersville Basic Nucleofector Kit for Primary Mammalian Smooth Muscle Cells
In addition to various cell type-specific kits for primary smooth muscle cells, we also provide a cell group-specific Basic Nucleofector Kit. This kit is suited to determine the optimal transfection conditions for any mammalian smooth muscle cell type that lacks a cell type specific Optimized Protocol for the Nucleofector 2b Device (or older generations I or II). In a first experiment a selection of different Nucleofector Programs is tested in combination with the Basic Nucleofector Solution for smooth muscle cells. Benefits:Optimization can be performed within 1 experiment Detailed protocols guiding through the optimization procedure Optional result fine tuning with help of our Scientific Support TeamTransfection efficiencies up to 95%Viabilities up to 96%Applications:Kit suited for smooth muscle cells from different mammalian species and various organs Already tested for porcine vascular smooth muscle cells and coronary artery smmoth muscle cells (CASMC)
Lonza Walkersville Cell Line Nucleofector Kit R
The Cell Line Nucleofector Kit R is one of our five Nucleofector Kits suited for transfecting cell lines in the Nucleofector 2b Device. This kit has been determined to be optimal for transfecting following cell types:B16-F0BJCOS-7HeLaHT-29Hut-78IMR-90KG-1L6LNCaPMDBKNIH/3T3NK-92PANC-1Sf9WI-38 The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.Benefits:High transfection efficiencies in difficult-to-transfect cell linesApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cells Low-throughput transfection in single cuvette formatContent:2.25 mL Nucleofector Solution R0.5 mL Supplement 130 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)25 Aluminum cuvette (100 µL)25 Single use pipettes
Lonza Walkersville Cell Line Nucleofector Kit C
The Cell Line Nucleofector Kit C is one of our five Nucleofector Kits suited for transfecting cell lines in the Nucleofector 2b Device. This kit has been determined to be optimal for transfecting following cell types:A2058CCRF-CEMMDA-MB-453MEG-01MG-63NCI-H1299 [H1299]NS0 (ECACC)SK-BR-3T2U266B1U-937The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.Benefits:High transfection efficiencies in difficult-to-transfect cell linesApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cells Low-throughput transfection in single cuvette formatContent:2.25 mL Nucleofector Solution C0.5 mL Supplement 230 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)25 Aluminum cuvette (100 µL)25 Single use pipettes
Expression Systems BestBac 2.0 Δ v-cath/chiA Baculovirus Cotransfection Kit
Expression Systems popular BestBac Linearized Baculovirus DNA backbone has been engineered to deliver highly efficient recombination with all polyhedron gene locus based transfer vectors.
Unmatched productivity and stability for BEVS
Improved production methodology can reduce need for plaque purification
Produces high titers of 5 E8 to 3 E9 IU/mL of recombinant baculovirus in P1 stock
Generates P1 stock as quickly as Bac-to-Bac and other systems
Each BestBac vial contains enough material for a minimum of five cotransfections
Suitable for other expression systems including BacMam™
Contains everything you need to perform cotransfections with your transfer vector. Included in the kit are:
• BestBac DNA
• pVL1393-lacZ control vector
• Transfection medium
Expres2 TR transfection reagent
To download Instructions for Use and/or COA please visit: www.expressionsystems.com
EZ BIOSYSTEMS INC HepG2/C3A Cell Avalanche® Transfection Reagent (human hepatocellular carcinoma cell) 0.5ml
HepG2/C3A Cell Avalanche® Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting HepG2/C3A cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for HepG2/C3A cells.
Lonza Walkersville Cell Line Nucleofector Kit T
The Cell Line Nucleofector Kit T is one of our five Nucleofector Kits suited for transfecting cell lines in the Nucleofector 2b Device. This kit has been determined to be optimal for transfecting following cell types:A431A549Caco-2CHO-K1D1 ORL UVAHT-1080NALM-6 (DSMZ)NRKP815RBL-2H3T84TF-1U-87 MGThe respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.Benefits:High transfection efficiencies in difficult-to-transfect cell linesApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cells Low-throughput transfection in single cuvette formatContent:2.25 mL Nucleofector Solution T0.5 mL Supplement 130 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)25 Aluminum cuvette (100 µL)25 Single use pipettes